ABSTRACT
OBJECTIVE: To establish a method for simultaneous determination of contents of β-pinene, linalool, L-camphor, L-borneol, β-caryophyllene and xanthoxylin in the oil of Blumea balsamifera. METHODS: GC method was adopted. The determination was performed on RTX-1701 capillary column (programmed temperature). The FID detector was controlled at 240 ℃. The inlet temperature was set at 240 ℃. The carrier gas was high-purity nitrogen 3 mL/min. The the sample size was 0.5 μL, and split ratio was 50 ∶ 1. RESULTS: The linear range of β-pinene, linalool, L-camphor, L-borneol, β-caryophyllene and xanthoxylin were 0.029 7-0.267 1 mg/mL (r=0.999 9), 0.024 3-0.218 9 mg/mL (r=0.999 9), 0.126 0-1.134 0 mg/mL (r=0.999 9), 0.217 2-1.954 8 mg/mL (r=0.999 9), 0.136 3-1.226 9 mg/mL (r=0.999 9), 0.044 5-0.400 3 mg/mL(r=0.999 5), respectively. The limits of quantitation were 0.028 5, 0.008 7, 0.018 6, 0.016 8, 0.014 5, 0.042 1 mg/mL; the limits of detection were 0.009 4, 0.002 9, 0.006 1, 0.005 5, 0.004 8, 0.013 9 mg/mL, respectively. RSDs of precision, stability, reproducibility and durability tests were all lower than 3%. The average recoveries were 98.13%-101.30%(RSD=1.20%,n=9),98.44%-101.81%(RSD=1.28%,n=9),98.26%-101.05%(RSD=1.19%,n=9),99.08%-101.58%(RSD=0.89%,n=9),98.66%-101.66%(RSD=1.17%,n=9),98.84%-103.60%(RSD=0.96%,n=9), respectively. The contents of 6 components in the sample were 14.552-46.766, 16.951-22.096, 80.597-113.115, 205.224-242.537, 47.761-135.697, 26.493-45.771 mg/g, respectively. CONCLUSIONS: The established method is simple, accurate, precise and reproducible, which can be used for simultaneous determination of contents of 6 components in the oil of B. balsamifera. It can provide reference for comprehensive evaluation and extraction technology study of the oil of B. balsamifera.
ABSTRACT
Aim To study the effects of L-borneol on the chloride channel and cell volume of human umbili-cal vein endothelial cells (HUVECs). Methods Whole-cell patch-clamp technique was used to record chloride currents. The expression of ClC-3 protein was down-regulated by siRNA interference technique. The cell volume was measured by dynamic image analysis. Results 20 nmol·L-1L-borneol significantly activa-ted chloride current in HUVEC (79.59 ± 4.90) pA/pF, which could be inhibited by chloride channel blockers,NPPB and DIDS. The outward current inhib-itory rate of NPPB was (95.57 ± 2.57)%, while that of DIDS was (97.28 ± 6.36)%. The chloride current activated by L-borneol significantly decreased after the silence of ClC-3 (27.03 ± 3.89) pA/pF. Cell volume was markedly reduced by L-borneol (14.38 ± 1.58)%,which was inhibited after NPPB appliance. Conclusion L-borneol can activate ClC-3 chloride channel in HUVECs, which induces Cl- outflow then cell volume decrease.
ABSTRACT
OBJECTIVE: To establish a GC method for simultaneous determination of camphor, isoborneol, L-borneol, β-caryo-phyllene and xanthoxylin in aifen, combined with hierarchical cluster analysis. METHODS: The GC analysis was carried out on HP-5 capillary column (30 m × 0.32 mm, 0.25 μm). Temperature programs; 90℃ (hold 2 min) programmed to 100℃ at 4℃ · min-1, then programmed to 160℃ (hold 6 min) at 20℃ · min-1. The detector was FID with 240℃. The inlet temperature was set at 240℃. The carrying gas was high-purity nitrogen (3.0 mL · min-1), split ratio was 50:1, injection volume was 0.5 μL. Cluster analysis was performed by SPSS16.0 software. RESULTS: The methodology validation for the assay of camphor, isoborneol, l-horneol, β-caryophyllene and xanthoxylin presented that they were in good liner correlation in the ranges of 0.02-0.32 (r=1.000 0), 0.01-0.08(r=1.000 0), 0.20-7.95(r=1.000 0), 0.01-0.40(r=1.0000), 0.08-0.67 mg · mL-1 (r=0.999 8), with the average recoveries (n=9) of 101.04% (RSD=1.04%), 99.08% (RSD=1.50%), 98.76% (RSD=1.67%), 99.85% (RSD=1.97%), 97.74% ( RSD=1.65%), respectively. The 24 batches of sample analyzed can be clustered into three classes according to the content of the five components. CONCLUSION: The developed method is simple, quick and accurate, which provides reference for the quality control of aifen.